Journal: Bioactive Materials
Article Title: Hydrogel delivering antifibrotic agent and nano-sonosensitizer enhances efficacy of sonodynamic therapy in osteosarcoma treatment
doi: 10.1016/j.bioactmat.2025.10.001
Figure Lengend Snippet: SIS3 reprograms CAFs (a) Schematic representation depicting the in vitro activation of NIH3T3 cells into CAFs using an OS-conditioned medium. (b) Western blotting is used to assess the expression levels of CAFs activation markers α-SMA and FAP. (c) Immunofluorescence (IF) staining is used to visualize the expression of CAFs activation markers α-SMA and FAP (scale bar: 25 μm). (d) IF staining is used to detect the expression of α-SMA and FAP in NIH3T3 cells, CAFs, and CAFs co-cultured with SIS3 (scale bar: 25 μm). (e) Statistical analysis of relative fluorescence intensity (FI), presented as mean ± SD (n = 3). (f) Schematic illustration of the TGF-β/SMAD3 signaling pathway. (g) Western blotting is used to evaluate the activation status of the TGF-β/SMAD3 signaling pathway in NIH3T3 cells, CAFs, and CAFs co-cultured with SIS3 and the statistical analysis of relative SMAD and p-SMAD expression, presented as mean ± SD (n = 3). (h) Representative images from collagen gel contraction assays on NIH3T3 cells, CAFs, and CAFs co-cultured with SIS3 and the statistical analysis of relative contraction degree, presented as mean ± SD (n = 3). (i) Representative images from wound healing assays conducted on NIH3T3 cells, CAFs, and CAFs co-cultured with SIS3 (scale bar: 200 μm) and the statistical analysis of relative wound healing degree, presented as mean ± SD (n = 3). (j) Representative images from transwell migration assays performed on NIH3T3 cells, CAFs, and CAFs co-cultured with SIS3 (scale bar: 100 μm) and the statistical analysis of the relative number of migrated cells, presented as mean ± SD (n = 3).
Article Snippet: The human OS cell line 143B, the mouse OS cell line K7M2, the mouse embryonic fibroblasts NIH3T3, and the human umbilical vein endothelial cells HUVEC were obtained from the American Type Culture Collection (ATCC, USA).
Techniques: In Vitro, Activation Assay, Western Blot, Expressing, Immunofluorescence, Staining, Cell Culture, Fluorescence, Migration
ATCC is a verified supplier 
ATCC manufactures this product 
